What is the reagent solution used in Cyanmethemoglobin method?

What is the reagent solution used in Cyanmethemoglobin method?

This procedure is based on the oxidation of hemoglobin and its derivatives (except sulfhemoglobin) to methemoglobin in the presence of alkaline potassium ferricyanide. Methemoglobin reacts with potassium cyanide to form cyanmethemoglobin, which has maximum absorption at 540 nm.

Which reagent is used for hemoglobin?

Drabkin′s reagent is used in measuring haemoglobin from blood samples. It comprises potassium ferricyanide, potassium cyanide and potassium dihydrogen phosphate as components. Potassium ferricyanide oxidizes haemoglobin to methemoglobin and then to cyanmethemoglobin.

Which of the following reagent has been used in Cyanmethemoglobin method for Hb estimation?

Cyanmethemoglobin Method The principle of this method lies in conversion of hemoglobin to cyanmethemoglobin by the addition of Potassium cyanide and ferricyanide whose absorbance is measured at 540 nm in a photoelectric calorimeter against a standard solution.

Why is Cyanmethemoglobin is the most preferred method?

The haemoglobin is estimated with the help of cyanmethemoglobin curve. The advantages of this method are i) error due to subjective visual matching is avoided as spectrophotometer is used and hence reading is precise and reliable, ii) measures all forms of haemoglobin except sulphaemoglobin.

What is the other name for the Cyanmethemoglobin method?

Cyanmethemoglobin (Hemoglobin-Cyanide) Method for Estimation of Hemoglobin.

Which is the quickest method of Hb estimation?

Direct cyanmethemoglobin method has been the gold standard for hemoglobin estimation and is cheap but time consuming.

What is the basic principle of Cyanmethemoglobin determination of hemoglobin?

The cyanmethemoglobin method works on the principle of conversion of hemoglobin to cyanmethemoglobin by the addition of potassium cyanide and ferricyanide whose absorbance is measured at 540 nm in a photoelectric calorimeter against a standard solution [16].

Which chemical is used for Hb test?

Automated hematology analyzers Hb determination is done by HiCN or the oxy-hemoglobin method. In the former, the blood specimen is diluted with a reagent containing ferricyanide and cyanide, which converts Hb to HiCN. The absorbance of the HiCN at 540 nm wavelength is then used for quantitation.

What is the quickest method of Hb estimation?

Direct cyanmethemoglobin method has been the gold standard for hemoglobin estimation but other methods like hemoglobin color scale, Sahli technique, Lovibond-Drabkin technique, Tallqvist technique, copper-sulfate method, HemoCue and automated haematology analyzers are also available.

What is the quickest method of haemoglobin estimation?

Direct cyanmethemoglobin method
Direct cyanmethemoglobin method has been the gold standard for hemoglobin estimation but other methods like hemoglobin color scale, Sahli technique, Lovibond-Drabkin technique, Tallqvist technique, copper-sulfate method, HemoCue and automated haematology analyzers are also available.

Is sulfhemoglobin irreversible?

Sulfhemoglobin is irreversible, lasting the lifetime of the erythrocyte, and sulfhemoglobin molecules cannot carry oxygen.

Which is the Colour of acid Haematin?

brown color
Thoroughly mix blood and acid using a fine glass rod (HCL will react with the haemoglobin and convert it into acid-haematin, which has a brown color).

How does cyanmethemoglobin work in a reagent?

In a reagent solution the ferrous ions (Fe2+) of hemoglobin are oxidized to the ferric (Fe3+) state by potassium ferricyanide to form methemoglobin. Methemoglobin subsequently reacts with the cyanide ions provided by potassium cyanide to form cyanmethemoglobin.

What kind of cyanide is used to measure hemoglobin?

Potassium ferricyanide transforms hemoglobin into methemoglobin, and methemoglobin combines with potassium cyanide to produce hemiglobincyanide (cyanmethemoglobin). This method is optional for estimation of hemoglobin and this method is recommended by the International Committee for Standardization in hemotology.

What kind of reagent is used to measure haemoglobin?

General description Drabkin′s reagent is used in measuring haemoglobin from blood samples. It comprises potassium ferricyanide, potassium cyanide and potassium dihydrogen phosphate as components. Potassium ferricyanide oxidizes haemoglobin to methemoglobin and then to cyanmethemoglobin.

What is the concentration of Drabkin’s hemoglobin reagent?

Product No. H7379 Hemoglobin human can be used by dissolving in Drabkin’s solution at a concentration of 0.7171 mg/ml. This will be equivalent to a 180 mg/ml Cyanmethemoglobin Standard Solution that is diluted 1:251 per our Datasheet. It is not necessary to use a Hemoglobin standard from the same species as is being tested.